Generator

Part:BBa_I744214:Design

Designed by: Peter Nguyen   Group: iGEM07_Rice   (2007-10-24)


TetR regulated LuxN-Tsr Chimeric Receptor D


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 161
    Illegal SapI.rc site found at 1224
    Illegal SapI.rc site found at 2271


Design Notes

The internal NdeI (CATATG) site of the Tsr C-terminal fragment 2 occuring at 772bp of the original protein receptor gene was mutated to (CACATG). This allowed the fusion of the LuxN and Tsr fragments by cloning in an NdeI site upstream at 646bp of Tsr, to test the effects of different fragment lengths.


Source

LuxN N-terminal domain is from Vibrio harveyi. Tsr C-terminal domain is from E.coli.

References